DNA Sequencing and PCR Markets

[Amy Cole <amy.cole@researchandmarkets.com>]

I enclose details of our latest DNA Sequencing and PCR report.

Genetic analysis has become one of the most important laboratory techniques in the life sciences. The massive effort to sequence the human genome is paralleled by projects to sequence the genomes of infectious organisms, research animals including fruit flies, worms and rats, and important food crops. It is estimated that over $4.5 billion was spent worldwide in 2003 on industrial and academic research to understand human gene function.  This report describes the worldwide market for DNA sequencing and analysis products, which consists of reagents, assays and other consumables. The total market for molecular diagnostics products of all kinds is an important part of the overall sequencing market, and is described in detail.

The emphasis in this report is on DNA sequencing reagents and equipment primarily used in the life sciences. Life scientists are spread throughout academia, in hospitals and medical schools, as well as in industry and government laboratories and private research foundations. Most often, they are working in the fields of molecular biology, cell biology, and biotechnology, as well as specialties such as bioinformatics, cancer research, proteomics, genetic disease, immunology, and microbiology. As a group, these life scientists most frequently use amplification techniques.The fastest growing category of custom laboratory services is qualitative PCR services.

Reagents and Equipment for use in DNA sequencing is analyzed worldwide with  the sequencing of DNA for pharmaceutical, biotech, industrial and diagnostic development,  growth in this sector is forecast, with total market size predicted through 2010.

The niche of chip-based sequencers is described and predictions for annual growth and market size are made to 2010. The market for protein sequencing instrumentation is developing more slowly, but is described in detail.

The leader in this business, the Applied Biosystems division of Applera Corporation achieves the majority of its revenues from DNA sequencing technologies. The company has installed over 10,000 automated sequencers in the past decade and has over 70% of the market. By mid-2003, PE had over 1700 orders for its $300,000 ABI PRISM 3700 DNA analyzer, or 1475 excluding those from its sister divion of Applera, Celera Genomics business unit. 

Applied Biosystems continues to strengthen its leadership in this field. Other leading players described include Amersham Pharmacia Biosystems, Beckman and their purchase of Genomyx and HP's with its extensive collaborative agreements with Chiroscience to develop MS sequencing technologies and with Affymetrix on the GeneArray system. 

Companies developing or marketing competitive DNA array technology include like Affymetrix, Agilent Technologies, Inc., Amersham Biosciences K.K., Applied Biosystems, Inc., Axon Instruments, Inc., BD Biosciences Clontech, CombiMatrix Corporation, Digital Gene Technologies, Inc., febit ag, Illumina, Inc., Lynx Therapeutics, Inc., Nanogen, Inc., NimbleGen Systems, Inc., Sequenom, Inc., Xeotron, Inc., and Visible Genetics Inc. (recently acquired by Bayer) are profiled, together with over 50 other players in the segment. 

The DNA/PCR kit market for the research segment is highly fragmented and full of competitors. The report outlines the largest players and estimated market share in this segment. The key bioassay technologies in the life sciences industry are summarized and described.

The purpose of this report is to describe the specific market segment of the in life sciences market DNA sequencing. This report includes all of the generally accepted DNA oriented analytical activities that are currently used today. It examines these measurement technology platforms and their reagents and supplies as utilized in research, biotech, pharmaceutical and health care. 

This report seeks to:

- Identify viable technology drivers through a comprehensive look at various platform technologies for molecular sequenching, including immunoassays electrophoretic methods, probe-based nucleic acid assays, microarrays, and non-PCR sequencing 

- Discover feasible market opportunities via an identification of high-growth applications in different sequencing areas, with a focus on the biggest and expanding markets new technology platforms. 

- Focus on global industry development through an in-depth analysis of the major world markets for molecular sequencing, including forecasts for growth.

This report concentrates on the molecular sequencing market segment in important worldwide markets such as the U.S., Japan and Europe, including Germany, the U.K., and other countries of the big five. It focuses primarily on the research, pharma and biotech market segment, and, separately, on a description of the instruments, reagents and supplies marketed by major companies in this segment. The report discusses the market size, growth rates, and market components for instruments and reagents, controls, and consumables used in this area.

For a complete index of this report click on http://www.researchandmarkets.com/reports/41864

Report Index:

1.Overview

1.1  Statement of Report
1.2  About This Report
1.3  Scope of the Report
1.4  Objectives
1.5  Methodology
1.6  Executive Summary

2. Introduction to DNA Sequencing and Amplification, and PCR Technology

2.1 Science Overview: Introduction to DNA and Sequencing
2.1.1 Types of Genetics Investigations
2.1.2 Proteomics
2.1.3 Products for the Genomics Market
2.2 The Impact of the Human Genome Project on Molecular Biology 
2.3 Impact of DNA Sequencing Techniques on Biotechnology, Pharmaceutical and Healthcare Industries
2.4 Molecular Diagnosis in the Postgenomic Era?the Role of SNPs 
2.5 Advances in Molecular Diagnostic Technologies 
2.6 The Use of Polymerase Chain Reaction Technology
2.7 Genetic Variability and Disease

3. Market Analysis of Polymerase Chain Reaction and DNA Amplification Products

3.1 Overview of the Sequencing and Amplification Market
3.1.1 Research Laboratories
3.1.1.1 Market Share of DNA Kit Manufacturers
3.1.2 Biotechnology
3.1.2.1 Competitive Situation
3.1.3 Pharmaceutical
3.1.3.1 Paradigm Shift in Drug Discovery and Development  
3.1.3.2 Systems Biology and the Need for More Effective Tools
3.1.3.3 Molecular Medicine and the New Therapeutics
3.1.3.4 Pharmacogenomics Using Genotyping and Phenotyping Clinical Biomarkers  
3.1.3.5 Competitive Situation
3.1.4 IVD Industry Nucleic Acid Test (NAT) Applications 
3.1.4.1 The Market
3.1.4.2 Market Share 
3.1.4.3 Growth Rate
3.1.4.4 Competitive Situation
3.1.4.5 DNA Kit Companies
3.1.4.6 Estimate of Units Sold
3.1.4.7 Pricing
3.2 Diagnostic Testing Using DNA
3.2.1 HIV
3.2.2 Hepatitis Serological markers and Nucleic Acid Testing
3.2.3 SARS virus
3.2.3.1 Microarray or DNA chip based tests
3.2.4 Cancer Detection
3.2.5 Microbiology Testing
3.2.6 Blood Screening
3.3 SNPs
3.3.1 The Market 
3.3.2 Market Influences and Forecast
3.4 Forensics Market
3.5 Target Selection 
3.6 Detection of Amplified DNA 
3.6.1 PCR
3.6.2 DNA Amplification Reagents Key Players and Market Share
3.7 New Developments in PCR
3.7.1 Ramification Amplication Method
3.7.2 Rolling Circle Amplification
3.8 Real Time Quantitative PCR 
3.9 Light Cycler PCR
3.10 Hot Start PCR
3.11 Ligation-Mediated PCR 
3.12 Molecular Beacons 
3.13 Amplification of Refractory Mutation System (ARMS) 
3.14 Market Size 1998-2009 & Growth Projections in DNA Amplification Instrumentation
3.14.1 U.S.
3.1.4.2 Far East
3.14.3 Europe
3.15 Market Share of Key Players in DNA Amplification Instrumentation
3.16 Competitive Situation 
3.16.1 Key Companies in PCR Amplification
3.16.2 Drivers of Competition 
3.16.3 Installed Base of PCR Instruments and ThermoCyclers
3.16.4 Pricing 
3.17 Isolation of DNA for PCR Profiling
3.17.1 Analysis by PCR-based DNA Typing
3.18 Rapid isolation of DNA for RFLP and PCR
3.18.1 DNA for PCR-STR Typing
3.19 Industrial Applications
3.19.1 The Market for Industrial Testing and Food Diagnostics
3.19.2Detection of H. pylori by PCR
3.19.3 E. coli 0157
3.19.4 Enteric Viruses
3.20 The Drivers of the Biotech Industry 
3.21 Biotechnology Instrumentation Outlook 
3.21.1 Products for the Genomics Market
3.21.2 Products for the Proteomics Market
3.21.3 Products for the Drug Metabolism and Pharmacokinetics Market
3.21.4 Cell Biology and Functional Proteomics Products
3.22 PCR/DNA Testing Market Analysis
3.21.1 Methods for DNA Detection and Gene Sequencing
3.21.2 Products & Applications 
3.21.3 Oncology
3.21.4 Toxicogenomics
3.21.5 Solution-Phase Multiplexing
3.21.6 Gene Expression Profiling
3.21.7 Proteomic Multiplex Assays
3.21.8 Gene Expression Monitoring Arrays
3.21.9 DNA Analysis Arrays

4. Use of Polymerase Chain Reaction and DNA Amplification Products 

4.1 PCR Components
4.1.1 Primers for PCR
4.1.2 Thermal Cyclers
4.2 Sources of DNA for PCR
4.3 Primers for PCR
4.4 Thermostable DNA Polymerases
4.5 PCR Protocol
4.6 Equipment for PCR
4.6.1 Microchip PCR Array Instrument 
4.6.1.1 Overview
4.6.1.2 Leading Companies
4.6.2 Automated Programmable Electronic Matrix (APEX) 
4.6.3 Microchip and DNA Chip Arrays
4.6.3.1 Gene Chip Usage in Molecular Diagnostics
4.6.3.2 Design of a Microarray
4.6.3.3 Sequence Chips
4.6.3.4 Gene Chips
4.6.3.5 DNA Analysis Arrays 
4.6.3.6 DNA Chip Sensors
4.6.4 Miniaturized Microfluidics Systems 
4.6.5 Microfluidic Automated DNA Analysis Using PCR 
4.6.6 Lab-On-A-Chip 
4.6.7 Chromosome on a Chip 
4.6.8 RNA Chip 
4.6.9 Protein Chip 
4.6.10 Applications of Protein Chip Technology 
4.7 FISH Amplification
4.8 Lab Chip Market Size
4.8.1 Market Size and Growth Projections
4.8.2 Market Share of Key Players 
4.8.3 Competitive Situation 
4.8.3.1 Key Companies in Microfluidics and Chips
4.8.3.2 Drivers of Competition 
4.8.3.3 Funding Sources 
4.8.3.4 Pricing 
	
5. Technical Trends in PCR 

5.1 Practical Applications of PCR
5.1.1 Overview
5.1.2 Proteomics
5.1.3 Bioinformatics
5.1.4 Genomics
5.1.5 Sequencing
5.1.6 Biotherapeutics
5.2 Quality Control Issues in PCR
5.3 Determining Length of cDNA Product
5.3.1 Electrochemical Detection of DNA 
5.3.2 New Technologies for Signal Amplification 
5.3.2.1 Dendritic Nucleic Acid (3 DNA) Signal Amplification 
5.3.2.2 Direct Signal Amplification Using the Invader Assay
5.3.3 Biosensor Technologies 
5.3.3.1 DNA Hybridization Biosensor Chips 
5.3.3.2 PNA Based Biosensors 
5.3.3.3 Surface Plasmon Resonance Biosensors 
5.4 Quantum Dot Technology 
5.5 Nucleic Acid Amplification Techniques 
5.6 Microfluidic Devices 
5.7 Genotyping Methods 
5.7.1 Use of DNA Sequencers 
5.7.2 Use of Microarrays 
5.8 Evolution of rapid point-of-care formats 
5.9 Development of SNP Genotyping on Very High Density Arrays
5.10 Protein Microarrays
5.11 RiboMaker Detection System

6. Trends in DNA Sequencing and Amplification 
6.1 Pharmacogenomics 
6.1.1 The Market 
6.1.1.1 Oncology
6.1.1.2 Toxigenomics
6.1.2 Market Influences and Forecast 
6.2 DNA Microarrays 
6.2.1 The Technique 
6.2.2 The Market 
6.2.3 Identification of Sequence SNPs 
6.2.4 Gene Expression Monitoring Arrays
6.3 Emerging Markets of DNA Arrays 
Pharmacogenomics 
Cancer 
Microbiology 
Human leukocyte antigen typing 
Neonatal screening  
6.6 Business Trends in the Industry 
6.6.2 Pharmaceutical Industry Trends 
6.6.3 Acquisitions, License Agreements, Partnerships 
6.6.4 Legal and Patent Developments
6.6.5 Ethical and legal issues related to the use of genetic information

7. New Products Under Development

7.1 Gene Expression Profiling
7.2 Proteomic Multiplex Assays
7.2.1 etag Chemistries
7.2.2 ArrayPlate Microarray-based Approach
7.3 Solution-Phase Multiplexing
7.4 RNAi 
7.4.1 The Role of RNAi in Drug Target Validation
7.4.2 Other Companies in the RNAi Market
7.5 Alternatives to PCR Amplification
7.5.1 Locked Nucleic Acid
7.5.2 Multiplexed Nuclease-Protection Assay and ArrayPlate

8. Corporate Directory 

Abbott Laboratories 
ACLARA BioSciences 
Advalytix
Affymetrix 
Agilent
Ambion 
Amersham Biosciences  
Applera: Applied Biosystems/Celera
Bayer Corporation 
Beckman Coulter 
Becton Dickinson and Company 
Biacore 
bioM?rieux 
Bio-Rad Laboratories 
BioTrove
Caliper Technologies 
Cellmark Diagnostics 
Cellomics 
Cepheid 
Chiron 
Ciphergen Biosystems  
Combimatrix Group
CuraGen 
Cytocell 
DakoCytomation A/S
diaDexus 
Digene Diagnostics Inc 
Enzo Diagnostics 
Exiqon 
Gene Logic 
Genisphere 
Genometrix 
Gen-Probe 
Genset 
Genzyme Genetics 
Genzyme Molecular Oncology 
Hamilton Thorne Biosciences
High Throughput Genomics
Hycor Biomedical, Inc.
Hyseq 
ID Biomedical 
IGEN International 
Illumina 
Incyte Genetics 
Incyte Pharmaceuticals 
Infectio Diagnostic Inc 
Innogenetics NV/SA 
InnoGenex 
Invitrogen
Interleukin
KREATECH Diagnostics 
Luminex Corp.
Lion Biosciences, AG
LI-COR 
Matritech
Meridian
Millennium Predictive Medicine 
MitoKor 
MWG Biotech 
Myriad Genetics 
Nanogen 
Nanosys
Nimblegen
Nova Molecular Inc 
Nymox
Oncor 
Orchid Biocomputer 
OrganonTeknika 
Ortho-Clinical Diagnostics 
Ostex
Pamgene
PE Biosystems 
PE Tropix 
PerSeptive Biosystems 
PharMingen 
PNA Diagnostics 
PPD 
PPGx 
PGL Professional Genetics Laboratory 
Promega 
Qiagen 
Quidel
Quantum Biotechnologies 
Roche Molecular Systems 
Sequenom 
Stratagen
Tepnel Life Sciences 
Third Wave Technologies 
TM Biosciences
Variagenics 
Visible Genetics 
Vysis 
453 Life Sciences (Curagen)

9.  Market Trends & Forecasts

9.1 Market Dynamics
9.2 Future Considerations for Molecular Diagnostics
9.3 How Genomics is Transforming Pharmaceutical R&D
9.4 Cancer Markers
9.5 Cytology Markers
9.6 RNA detection
9.7 Diagnosis of Inherited disease
9.8 Ethical Considerations
9.9 NAT and personalized medicine 
9.10 Three Growth Areas of Molecular Diagnostics
9.11 PNA Probes
9.12 Rolling Circle Amplification
9.13 Ramification Amplification Method
9.14 Invader Assays/Third Waves Technologies
9.15 Molecular Beacons

Glossary

List of Tables	

Table 1 Global Market for DNA Sequencing Analysis ? 28
Table 2 DNA Sequencing Market Players and Related Revenue ? 29
Table 3 Reagents and Equipment Sales for Use in DNA Sequencing Worldwide ? 46-47
Table 4 The Worldwide Market For Protein Sequencing Instrumentation ? 49
Table 5 Global Market For Tools and Consumables Used in Drug Discovery And Development, Clinical Diagnostics and Biomedical Research ? 51
Table 6 Estimated Market Share and Major Competitors in the DNA/PCR Kit Research Market Segment in the U.S. 2002 ? 53
Table 7 Key Bioassay Technologies in the Life Sciences Industry ? 55 
Table 8 Global Market for Molecular Diagnostic Testing ? 65 
Table 9 Global Market for Nucleic Acid Testing ? 65 
Table 10 Summary of Molecular Diagnostic Testing 2001 ? 67 
Table 11 Annual U.S. Market for DNA Probe Diagnostics ? 68 
Table 12 Annual Worldwide Market for DNA Probe Diagnostics ? 69 
Table 13 Estimated Market Share and Major Competitors in the DNA/PCR Kit Diagnostics Market Segment in the U.S. 2002 ? 72 
Table 14 DNA Probe Market Segments for the U.S. Market ? 73 
Table 15 Companies Marketing Products Using DNA Probes ? 78 
Table 16 Number of Units Sold Worldwide of DNA Probe Kits ? 80 
Table 17 Summary of Assays for HIV Viral Load Testing ? 85-86
Table 18 Global Market for HIV Diagnostic Testing ? 88 
Table 19 U.S. Market for HIV Diagnostic Testing ? 88 
Table 20 Lower Detection Limits of HBV DNA Assays ? 89 
Table 21 Gen-Probe Clinical Diagnostic Products for the Detection of Viral Microorganisms ? 94 
Table 22 Global Market for SARS Diagnostic Testing ? 98 
Table 23 U.S. Market for SARS Diagnostic Testing ? 98 
Table 24 Global Market for Molecular Diagnostic Cancer Testing ? 101 
Table 25 Annual Test Volume for Infectious Disease NAT Testing ? 102 
Table 26 World Market Dollar Volume of NAT STD Testing ? 103 
Table 27 U.S. Market Dollar Volume of NAT STD Testing ? 103 
Table 28 U.S. Market Share of NAT STD Testing ? 104 
Table 29 Gen-Probe Clinical Diagnostic Products for the Detection of Non-Viral Microorganisms ? 104-109
Table 30 Global Market for Blood Screening Diagnostic Testing ? 112 
Table 31 U.S. Market for Blood Screening Diagnostic Testing ? 112 
Table 32 Gen-Probe Blood Screening Products ? 114 
Table 33 Leading Players in SNP Genotyping ? 120 
Table 34 Global Market for SNP Genotyping ? 120 
Table 35 DNA Analysis Market Competitors ? 125 
Table 36 Leading Companies in Medical Utility of SNPs ? 125 
Table 37 U.S. Market for Forensic Diagnostic Testing ? 127 
Table 38 Properties Of Various Nucleic Acid Amplification Techniques ? 132 
Table 39 Frequency Usage of DNA Amplification Applications ? 133-134
Table 40 Hot-start PCR Leaders and U.S. Market Share 2002 ? 139 
Table 41 Companies Licensed to Sell Custom Molecular Beacon Probes ? 142 
Table 42 U.S. Market Size 1998-2008 & Growth Projections in DNA Amplification Instrumentation ? 144 
Table 43 Far East Market Size 2000-2008 & Growth Projections in DNA Amplification Instrumentation ? 145 
Table 44 Market Share of Key Players in DNA Amplification ? 146 
Table 45 Market Share of Key Players in DNA Amplification Thermostable Enzymes ? 147 
Table 46 Real Time PCR Users Type of DNA Amplification MarketShare ? 147 
Table 47 Nucleic Acid Amplification Methods ? 148 
Table 48 Amplification Reagent Product Description ? 149 
Table 49 Installed Base of  Real Time PCR Instruments and ThermoCyclers ? 150 
Table 50 Leading Manufacturers of Themal Cyclers and Market Share ? 151 
Table 51 Standard Prep Pricing ? 152 
Table 52 Small Target Prep Pricing ? 152-153 
Table 53 Hybridization Pricing ? 153 
Table 54 Analysis ? 153 
Table 55 Gene Chip Pricing ? 155-156 
Table 56 Affymetrix Made-To-Order Arrays ? 157 
Table 57 U.S. Market for Food Screening Diagnostic Testing ? 160 
Table 58 Leading Manufacturers of Thermal Cyclers and Market Share ? 184 
Table 59 Thermo Electron Molecular Biology Thermal Cyclers ? 186 
Table 60 Companies Developing Or Marketing Potentially Competitive DNA Array        ? 202 
Table 61 Global Market for DNA Chips 2000-2006 ? 203 
Table 62 Worldwide Market for Microfluidic Devices ? 209 
Table 63 Lab Chip Products ? 243-244 
Table 64 Automated Chip Systems ? 246-247
Table 65 Chip Application Development Systems ? 250 
Table 66 The Worldwide Protein Biochip Market ? 251 
Table 67 Global Protein Chip Market ? 261 
Table 68 RiboMaker? assays to be introduced for Cancer and Pathogen Detection ? 295
Table 69 Products Marketed By Abbott In The Diagnostic Market ? 327 
Table 70 Summary of Selected Affymetrix Expression Profiling Customers ? 338-339
Table 71 Summary of Affymetrix Selected Collaborators and Licensees ? 346-347
Table 72 Amersham Biosciences -- Protein Separations and Discovery Systems ? 363 
Table 73 Gen-Probe PACE Product Sales as a Percentage of Total Company Revenue     ? 442 
Table 74 IGEN International Product Line ? 460-462
Table 75 Nanogen Product Sales ? 496 
Table 76 Qiagen Sales by Country 2002 and 2003 ? 513-514
Table 77 Companies Licensed to Sell Custom Molecular Beacon Probes ? 552-553

List of Figures

Figure 1. Segment of Double-Stranded DNA Showing the Base Pair Relationship ? 19
Figure 2  Overall Structure of Nucleic Acid Testing Market - 62 
Figure 3  Formation of Enzyme-DNA Complex Analyte ? 136 
Figure 4  Cepheid?s I-CORE Module ? 233 
Figure 5 Diversa ?s GigaMatrix well plate ? 235 
Figure 6 Illustration of SNP Genotyping ? 258 
Figure 7 The Layered Structure of the 3DNA Dendrimer
Figure 8 Example of Protein or Antibody (Ab) Array (Hypomatrix) ? 293


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Amy Cole
Senior Manager
Research and Markets Ltd
amy.cole@researchandmarkets.com

REPORT DATA SUMMARY:
DNA Sequencing and PCR Markets
Publisher Name: TriMark Publications
Date Published: 01/01/2004
Number of Pages: 558
Category: Biotechnology
URL: www.researchandmarkets.com/reports/41864

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