Introduction

Mechanism
Novel
Threonine 1 is nucleophile
N-terminal nucleophiles
Catalytic tetrad:
Thr1, amino terminus, Lys 14, Glu 33

Inhibited by many electrophilic inhibitors
aldhydes, boronic esters, alpha-keto peptides
Several serine protease inhibitors do not affect it
PMSF, leupeptin, aprotinin
Specific new inhibitors
lactacystein (beta-lactones), epoxyketone peptides

Peptide product length of 8-10 amino acids
"molecular ruler"