Materials

Overexpression system
Obtained from Baumeister (Max-Planck, Germany)
E. coli overexpression system
Mature, processed beta-, alpha-subunits
6 His tagged beta-subunits
NiNTA purification

Typical yield: 16 mg/enzyme per four liters of culture

Verification of expression
TEM verified assembly of particles
Mass spectrometry verified intact proteins