Protein Digestion

Overview of aims
Can 20S proteasome digest proteins?
Does the rate compare to better characterized proteases?
What is the effect of activators on protein hydrolysis?

Methods
Monitor Trp fluoresence (280 nm, 305 nm) of substrate protein
20S proteasome lacks any Trp residues
no quantification
verified with SDS-PAGE
Destabilized protein substrates
carboxymethylated, acetylated
Standard assay conditions